Core Technology

Randolph, T. W., M. Seefeldt, et al. (2002). "High hydrostatic pressure as a tool to study protein aggregation and amyloidosis." Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology 1595(1-2): 224-234.

Qoronfleh, W., L. K. Hesterberg, et al. (2007). "Confronting high-throughput protein refolding using high pressure and solution screens." Protein Expression and Purification 55: 209-224.

Seefeldt, M. B., C. Crouch, et al. (2007). "Specific volume and adiabatic compressibility measurements of native and aggregated recombinant human interleukin-1 receptor antagonist: Density differences enable pressure-modulated refolding." Biotechnology And Bioengineering 98(2): 476-485.

Seefeldt, M. B., J. Ouyang, et al. (2004). "High-pressure refolding of bikunin: Efficacy and thermodynamics." Protein Science 13(10): 2639-2650.

St. John, R. J., J. F. Carpenter, et al. (1999). "High pressure fosters protein refolding from aggregates at high concentrations." Proceedings of the National Academy of Sciences of the United States of America 96(23): 13029-13033.

Crisman, R. L. and T. W. Randolph (2008). "Refolding of Proteins form Inclusion Bodies is Favored by a Diminsished Hydrophobic Effect at Elevated Pressure." Biotechnology and Bioengineering In Press.

US Patents: 6489450 and 7064192

European patents issued and multiple patents pending.

 

Enabling High Pressure Refold Examples

Arana, M. E., G. K. Powell, et al. (2010). "Refolding active human DNA polumerase v from inclusion bodies." Protein Expression and Purification In press.

Chura-Chambi, R. M. e. a. (2008). "Refolding of endostatin from inclusion bodies using high hydrostatic pressure." Analytical Biochemistry 379: 32-39.

Lee, S. H., J. F. Carpenter, et al. (2006). "Effects of solutes on solubilization and refolding of proteins from inclusion bodies with high hydrostatic pressure." Protein Science 15(2): 304-313.

Saio, T., M. Yokochi, et al. (2010). "PCS-based structure determination of protein-protein complexes." Journal of Biomolecular NMR 46(4): 271-280.

Schoner, B. E., K. S. Brandt, et al. (2005). "Reconstitution of nuclear receptor proteins using high pressure refolding." Molecular Genetics and Metabolism 85: 318-322.

Shieh, H. S., K. J. Mathis, et al. (2008). "High resolution crystal structure of the catalytic domain of ADAMTS-5 (aggrecanase-2)." Journal of Biological Chemistry 283(3): 1501-1507.

 

Aggregate Dissociation and Elimination for Reduced Immunogenicity

Cleland, J. L., M. S. Rosendahl, et al. (2009). BaroFeron, a More Bioavailable and Less Immunogenic IFN Beta Product. The Consortium of Multiple Sclerosis Centers Annual Meeting, Denver, CO.

Fradkin, A. H., J. F. Carpenter, et al. (2009). "Immunogenicity of aggregates of recombinant human growth hormone in mouse models." Journal of Pharmaceutical Sciences 98(9): 3247-3264.

Seefeldt, M. B., M. S. Rosendahl, et al. (2009). "Application of High Hydrostatic Pressure to Dissociate Aggregates and Refold Proteins." Current Pharmaceutical Biotechnology 10: 447-455.

 

High Pressure Crystallization

Crisman, R.L., Randolph, T.W.,  (2010). Crystallization of Growth Hormone at Elevated Pressures: Pressure Effects on PEG-Induced Excluded Volume Interactions.

 

High Pressure PEGylation

Seefeldt, M. B., M. S. Rosendahl, et al. (2009). "Application of High Hydrostatic Pressure to Dissociate Aggregates and Refold Proteins." Current Pharmaceutical Biotechnology 10: 447-455.