BaroFold Publications
Confronting High-throughput Protein Refolding Using High Pressure and Solution Screens”, M.W. Qoronfleh, L.K Hesterberg, M.B. Seefeldt, Protein Expression and Purification. Protein Expr Purif. 2007 Oct;55(2):209-24. Epub 2007 Jun 2. Click here for reference information.
Pressure-Modulated Refolding of a Malaria Vaccine Candidate (Pfs48) from Inclusion Bodies, M.B. Seefeldt, A. Saul, J. F. Carpenter, L.K. Hesterberg, T.W. Randolph, Manuscript in Preparation.
Protein disaggregation and refolding using high hydrostatic pressure. Phelps, DJ and L.K. Hesterberg (2007). J Chem Technol Biotechnol 82 610-613. Click here for reference information.
Alternatives to Chromatographic Separations. J. Thommes and M. Etzel. (2007) Biotechnol. Prog. 23 42-45. Click here for reference information.
“High-pressure studies on protein aggregates and amyloid fibrils” Kim Y.S, T. Randolph, Seefeldt, MB, Carpenter J., (2006). Methods in Enzymology 413 237-253. Click here for reference information.
High-Hydrostatic pressure refolding of proteins." Hesterberg, L. K., M. B. Seefeldt, et al. (2005). Genetic Engineering News 25(4): 46-47.
High Pressure Disaggregation and Folding of Recombinant Proteins: Examples and Production Cost Comparison. J.F. Carpenter, L.K. Hesterberg, and T.W. Randolph. (2005) BioProcess International 3 36-44.
CU Publications
“Specific volume and adiabatic compressibility measurements of native and aggregated recombinant human interleukin 1 – receptor antagonist : Density differences enable pressure-modulated refolding” M. B. Seefeldt, C. Crouch, et al. Biotechnology and Bioengineering, Accepted, In Press.". Biotechnology and Bioengineering 98 (2): 476-485 Oct 1, 2007. Click here for reference information.
"High-pressure studies of aggregation of recombinant human interleukin-1 receptor antagonist: Thermodynamics, kinetics, and application to accelerated formulation studies." Seefeldt, M. B., Kim Y. S., et al. (2005). Protein Science 14(9): 2258-2266. Click here for reference information.
“High hydrostatic pressure as a tool to study protein aggregation and amyloidosis.,” Randolph T., Seefeldt M., & Carpenter J., Biochimica et Biophysica Acta, 2002. Click here for reference information.
"High-pressure refolding of disulfide-cross-linked lysozyme aggregates: Thermodynamics and optimization." St. John, R. J., J. F. Carpenter, et al. (2002). Biotechnology Progress 18(3): 565-571. Click here for reference information.
"High pressure refolding of recombinant human growth hormone from insoluble aggregates - Structural transformations, kinetic barriers, and energetics." St. John, R. J., J. F. Carpenter, et al. (2001). Journal of Biological Chemistry 276(50): 46856-46863. Click here for reference information.
"High pressure fosters protein refolding from aggregates at high concentrations." St. John, R. J., J. F. Carpenter, et al. (1999). Proceedings of the National Academy of Sciences of the United States of America 96(23): 13029-13033. Click here for reference information.
BaroFold and CU
“The impact of aggregate formation conditions and the “refolding window” on the high pressure refolding of protein aggregates” – Estimated submission 2 months
“High pressure protein stability: A backbone-based theory” - Estimated submission 3 months
“Pressure effect on rhGH solubility and crystallization” – Estimated submission 9 months
Oral Presentations at Conferences (2006 and later)
- 2008 CHI-PEPtalk Protein meetings – Invited speaker
- 2008 EUFEPS - Dev. of Safe Protein Therapeutics - Invited Speaker
- BioNetwork East - Invited Speaker
- AAN Chicago - Invited Speaker
- BIO 2008 - Invited Speaker
- Current Challenges in Protein Therapeutics
- International BioProcess Conference
- Difficult to Express Protein Conference
- American Institute of Chemical Engineers National Conference (AiChe)
- ACS; BIO session
- 2007 Difficult to Expression Protein Conference
- Pharmaceutical Sciences World Conference
- BIO 2007 – Session on Immunogenicity and Aggregation
- 2006 CHI-PEPtalk Protein Meeting
Poster Presentations at Conferences
- AAN Chicago
- Follow-On Biologics Conference
- Protein Stability Conference
- National Protein Society Conference
- American Association of Pharmaceutical Scientist National Conference
- American Association of Pharmaceutical Scientist Biotechnology Conference
SELECTED PUBLICATIONS:
High Pressure Refolding
Seefeldt, M. B., J. Ouyang, et al. (2003). "High-pressure refolding of bikunin protein aggregates: efficacy and thermodynamics." Biochemistry 42(28): 8643-8643. Click here for reference information
St John, R. J., J. F. Carpenter, et al. (2001). "High pressure refolding of recombinant human growth hormone from insoluble aggregates - Structural transformations, kinetic barriers, and energetics." Journal of Biological Chemistry 276(50): 46856-46863. Click here for reference information.
St John, R. J., J. F. Carpenter, et al. (1999). "High pressure fosters protein refolding from aggregates at high concentrations." Proceedings of the National Academy of Sciences of the United States of America 96(23): 13029-13033. Click here for reference information.
St John, R. J., J. F. Carpenter, et al. (2002). "High-pressure refolding of disulfide-cross-linked lysozyme aggregates: Thermodynamics and optimization." Biotechnology Progress 18(3): 565-571. Click here for reference information.
Schoner, B. E., K. S. Brandt, et al. (2005). "Reconstitution of Nuclear Receptor Proteins using High Pressure Refolding." Molecular Genetics and Metabolism 85: 318-322. Click here for reference information.
Pressure Effects on Protein Structure
Hawley, S. A. (1971). "Reversible Pressure-Temperature Denaturation of Chymotrypsinogen." Biochemistry 10(13): 2436.
Brandts, J. F., R. J. Oliveira, et al. (1970). "Thermodynamics of Protein Denaturation - Effect of Pressure on Denaturation of Ribonuclease-A." Biochemistry 9(4): 1038
Randolph, T. W., M. Seefeldt, et al. (2002). "High hydrostatic pressure as a tool to study protein aggregation and amyloidosis." Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology 1595(1-2): 224-234. Click here for reference information.
Royer, C. A. (2002). "Revisiting volume changes in pressure-induced protein unfolding." Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology 1595(1-2): 201-209. Click here for reference information.
Smeller, L. (2002). "Pressure-temperature phase diagrams of biomolecules." Biochimica Et Biophysica Acta-Protein Structure and Molecular Enzymology 1595(1-2): 11-29. Click here for reference information.
Gross, M. and R. Jaenicke (1994). "Proteins under Pressure - the Influence of High Hydrostatic- Pressure on Structure, Function and Assembly of Proteins and Protein Complexes." European Journal of Biochemistry 221(2): 617-630. Click here for reference information.
Balny, C. (2002). "High pressure and protein oligomeric dissociation." High Pressure Research 22(3-4): 737-741.
Processing / Economic Advantages of PreEMT™ Refolding
High Pressure Disaggregation and Folding of Recombinant Proteins: Examples and Production Cost Comparison. J.F. Carpenter, L.K. Hesterberg, and T.W. Randolph. (2005) BioProcess International 3 36-44.
Clark, E. D. (2001). "Protein refolding for industrial processes." Current Opinion in Biotechnology 12(2): 202-207. Click here for reference information.
Pressure Accelerated formulation/Stability studies
Seefeldt, M. B., Y. S. Kim, et al. (2005). "High-pressure studies of aggregation of recombinant human interleukin-1 receptor antagonist: Thermodynamics, kinetics, and application to accelerated formulation studies." Protein Science 14(9): 2258-2266. Click here for reference information.
Roberts, C. J., R. T. Darrington, et al. (2003). "Irreversible aggregation of recombinant bovine Granulocyte-Colony Stimulating Factor (bG-CSF) and implications for predicting protein shelf life." Journal of Pharmaceutical Sciences 92(5): 1095-1111. Click here for reference information.
Roberts, C. J. (2003). "Kinetics of irreversible protein aggregation: Analysis of extended Lumry-Eyring models and implications for predicting protein shelf life." Journal of Physical Chemistry B 107(5):
1194-1207.
Protein Aggregation
Chi, E. Y., S. Krishnan, et al. (2003). "Physical stability of proteins in aqueous solution: Mechanism and driving forces in nonnative protein aggregation." Pharmaceutical Research 20(9): 1325-1336. Click here for reference information.
Cleland, J. L., M. F. Powell, et al. (1993). "The Development of Stable Protein Formulations - a Close Look at Protein Aggregation, Deamidation, and Oxidation." Critical Reviews in Therapeutic Drug Carrier Systems 10(4): 307-377. Click here for reference information.
